Reliable measurement of multiplexed biomarker panel of RNA transcripts
نویسندگان
چکیده
منابع مشابه
Simultaneous Multiplexed Measurement of RNA and Proteins in Single Cells.
Significant advances have been made in methods to analyze genomes and transcriptomes of single cells, but to fully define cell states, proteins must also be accessed as central actors defining a cell's phenotype. Methods currently used to analyze endogenous protein expression in single cells are limited in specificity, throughput, or multiplex capability. Here, we present an approach to simulta...
متن کاملMultiplexed tissue biomarker imaging.
Description of the technology The detection of structural and functional proteins in cells within the tumor microenvironment in tissue samples is achieved by immunolabeling with specific antibodies. These target proteins are visualized with the subsequent application of either an enzymatic reaction that induces chromogen precipitation at the site of antibody-antigen binding (immunoenzyme method...
متن کاملprocalcitonin, a reliable biomarker in management of sepsis
procalcitonin is widely used as a biomarker for diagnosis and prognosis for sepsis and infection as well as monitoring the response to antibiotic therapy. in this study we systematically reviewed the value of procalcitonin in diagnosis, treatment, and prognosis in patients with sepsis or infection.
متن کاملRapid and label-free nanomechanical detection of biomarker transcripts in human RNA.
The availability of entire genome sequences has triggered the development of microarrays for clinical diagnostics that measure the expression levels of specific genes. Methods that involve labelling can achieve picomolar detection sensitivity, but they are costly, labour-intensive and time-consuming. Moreover, target amplification or biochemical labelling can influence the original signal. We h...
متن کاملNoncoding RNA transcripts.
Recent analyses of the human genome and available data about the other higher eukaryotic genomes have revealed that, in contrast to Eubacteria and Archaea, only a small fraction of the genetic material (ca 1.5%) codes for proteins. Most of genomic DNA and its RNA transcripts are involved in regulation of gene expression, which can be exerted at either the transcriptional level, controlling whet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2016
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2016.05.004